【文獻(xiàn)標(biāo)題】Prenatal nicotine exposure intergenerationally programs imperfect articular cartilage via histone deacetylation through maternal lineage
【作者】Zhe Xie, Zhe Zhao, Xu Yang，et.al
【作者單位】武漢大學(xué)中南醫(yī)院（Zhongnan Hospital of Wuhan University）
【文獻(xiàn)中引用產(chǎn)品】
【關(guān)鍵詞】Nicotine prenatal exposure; Articular cartilage; Transforming growth factor β; Histone
【DOI】doi:10.1016/j.taap.2018.03.018
【影響因子(IF)】3.94
【出版期刊】《Toxicology ａnd Applied Pharmacology》
【產(chǎn)品原文引用】
Chemicals ａnd reagents
Nicotine (CAS no. 54-11-5) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Safranin O (CAS no. 477-73-6) and fast green (CAS no. 2353-45-9) were purchased from Hengyuan Biotech (HengYuan Biological Technology Co., Ltd, Shanghai, China). Trizol reagent kits were obtained from Omega BioTek (Doraville, GA, USA). Exscript RT reagent kit ａnd SYBR(R) Premix Ex TaqTM were obtained from TaKaRa Biotechnology (Dalian, China). Oligonucleotide primers for analyzed genes were synthesized by Sangon Biotech (Shanghai, China). DMEM/F12 (1:1) ａnd fetal bovine serum were supplied by Gibco (St. Louis, MO, USA). Polyclonal antibodies for COL2A1, SOX9, TGFβ, TGFβ receptor 1 (TGFβR1) ａnd Smad2/3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Other chemicals ａnd agents were of analytical grade.
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