【文獻(xiàn)標(biāo)題】LncRNA MEG3 inhibits the infammatory response of ankylosing spondylitis by targeting miR‑146a

【作者】Yehong Li，Shanshan Zhang，Cunxin Zhang，et.al

【作者單位】濟寧市第人民醫(yī)院（Jining No.1 People’s Hospital）

【文獻(xiàn)中引用產(chǎn)品】

人白細(xì)胞介素-1β（IL-1β）elisa試劑盒

人白細(xì)胞介素-6（IL-6）ELISA試劑盒

人腫瘤壞死因子-α(TNF-α)ELISA試劑盒

【關(guān)鍵詞】Ankylosing spondylitis，MEG3，miR-146a，Infammatory response，Cytokines

【DOI】https://doi.org/10.1007/s11010-019-03681-x

【影響因子(IF)】5.10

【出版期刊】《Molecular ａnd Cellular Biochemistry》

【產(chǎn)品原文引用】

Enzyme‑linked immunosorbent assay (ELISA)

The levels of infammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), ａnd tumor necrosis factor-α (TNF-α) were detected by ELISA kit (Hengyuan, Shanghai, China). In short, the samples to be tested ａnd the standard samples were added to the corresponding enzyme plate ａnd cultured at 37 ℃ for 1 h. After washing with PBS, the chromogenic antibody was added to the enzyme plates ａnd continued incubation for 30 min. Finally, the termination solution was added to terminate the reaction. OD value at 450 nm was measured by a microplate reader (Thermo Fisher Scientifc, Rockford, IL, USA).

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原創(chuàng)作者：上海恒遠(yuǎn)生物技術(shù)發(fā)展有限公司